human cell line t 84 Search Results


human cell line t 84  (ATCC)
96
ATCC human cell line t 84
Human Cell Line T 84, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dulbecco's modified eagle medium (dmem)-f12 medium  (Thermo Fisher)
90
Thermo Fisher dulbecco's modified eagle medium (dmem)-f12 medium
Dulbecco's Modified Eagle Medium (Dmem) F12 Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dmem/f-12  (Mediatech)
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Mediatech dmem/f-12
Dmem/F 12, supplied by Mediatech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sodium pyruvate  (Thermo Fisher)
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Thermo Fisher sodium pyruvate
Sodium Pyruvate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dmem/f-12  (Cellgro)
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Cellgro dmem/f-12
Dmem/F 12, supplied by Cellgro, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2.5 mm l-glutamine  (Thermo Fisher)
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Thermo Fisher 2.5 mm l-glutamine
2.5 Mm L Glutamine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dmem/ham's f-12 medium (50:50 mix)  (Mediatech)
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Mediatech dmem/ham's f-12 medium (50:50 mix)
Dmem/Ham's F 12 Medium (50:50 Mix), supplied by Mediatech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ham's f-12 medium (dmem f-12 medium  (Millipore)
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Millipore ham's f-12 medium (dmem f-12 medium
Ham's F 12 Medium (Dmem F 12 Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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direct camp cgmp eia kits  (Enzo Biochem)
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Enzo Biochem direct camp cgmp eia kits
Mouse serum antibody in vitro neutralization activities against adherence of K88 fimbrial or F18 fimbrial bacteria and enterotoxicity of STa and CT toxins. (A) Serum samples from mice immunized with PWD MEFA, PWD MEFA adjuvanted with dmLT, F18 FedF recombinant protein (as the positive control), or PBS as the control in adherence inhibition against F18 fimbrial bacteria 8516 to IPEC-J2 pig intestinal cells. (B) Serum samples from mice immunized with PWD MEFA, PWD MEFA adjuvanted with dmLT, K88 FaeG recombinant protein (as the positive control), or PBS as the control in adherence inhibition against K88+ ETEC strain 3030-2 to IPEC-J2 cells. The numbers of adherent bacteria (CFU) were converted to percentages, with CFU from cells treated with the control serum at 100%. (C) Mouse serum samples from two immunized groups (PWD MEFA and PWD MEFA with dmLT adjuvant) or the control in prevention of STa toxin from stimulation of <t>cGMP</t> in T-84 cells to show antibody neutralization activity against STa enterotoxicity. T-84 cells incubated with 2 ng STa toxin premixed with PBS, control mouse serum, or serum from each immunized group were measured for intracellular cGMP levels (pmol/ml) by using a cGMP <t>EIA</t> kit (Enzo Life). (D) Mouse serum samples from the two immunized groups (PWD MEFA and PWD MEFA with dmLT adjuvant) or the control in prevention of CT (LT homologue) from stimulation of cAMP in T-84 cells to show antibody neutralization activity against CT enterotoxicity. T-84 cells incubated with 10 ng CT toxin premixed with PBS, control mouse serum, or each immunized mouse serum were measured for intracellular cAMP levels (pmol/ml) by using a cAMP EIA kit (Enzo Life). ***, P < 0.001; **, P < 0.01.
Direct Camp Cgmp Eia Kits, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eia camp elisa kit  (Assay Designs Inc)
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Assay Designs Inc eia camp elisa kit
Mouse serum antibody in vitro neutralization activities against adherence of K88 fimbrial or F18 fimbrial bacteria and enterotoxicity of STa and CT toxins. (A) Serum samples from mice immunized with PWD MEFA, PWD MEFA adjuvanted with dmLT, F18 FedF recombinant protein (as the positive control), or PBS as the control in adherence inhibition against F18 fimbrial bacteria 8516 to IPEC-J2 pig intestinal cells. (B) Serum samples from mice immunized with PWD MEFA, PWD MEFA adjuvanted with dmLT, K88 FaeG recombinant protein (as the positive control), or PBS as the control in adherence inhibition against K88+ ETEC strain 3030-2 to IPEC-J2 cells. The numbers of adherent bacteria (CFU) were converted to percentages, with CFU from cells treated with the control serum at 100%. (C) Mouse serum samples from two immunized groups (PWD MEFA and PWD MEFA with dmLT adjuvant) or the control in prevention of STa toxin from stimulation of <t>cGMP</t> in T-84 cells to show antibody neutralization activity against STa enterotoxicity. T-84 cells incubated with 2 ng STa toxin premixed with PBS, control mouse serum, or serum from each immunized group were measured for intracellular cGMP levels (pmol/ml) by using a cGMP <t>EIA</t> kit (Enzo Life). (D) Mouse serum samples from the two immunized groups (PWD MEFA and PWD MEFA with dmLT adjuvant) or the control in prevention of CT (LT homologue) from stimulation of cAMP in T-84 cells to show antibody neutralization activity against CT enterotoxicity. T-84 cells incubated with 10 ng CT toxin premixed with PBS, control mouse serum, or each immunized mouse serum were measured for intracellular cAMP levels (pmol/ml) by using a cAMP EIA kit (Enzo Life). ***, P < 0.001; **, P < 0.01.
Eia Camp Elisa Kit, supplied by Assay Designs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eia cgmp elisa kit  (Assay Designs Inc)
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Assay Designs Inc eia cgmp elisa kit
Supernatants of overnight grown culture (with an equal amount of cells) from each constructed strain were incubated with 1–2×10 5 T-84 cells. Panel A: Intracellular <t>cGMP</t> levels were measured using an <t>EIA</t> cGMP kit (Assay Designs), with 2 ng purified STa (from Robertson laboratory) was used as a positive control. Panel B: Intracellular cAMP levels were measured using an EIA cAMP kit with 10 ng CT as a positive control.
Eia Cgmp Elisa Kit, supplied by Assay Designs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Mouse serum antibody in vitro neutralization activities against adherence of K88 fimbrial or F18 fimbrial bacteria and enterotoxicity of STa and CT toxins. (A) Serum samples from mice immunized with PWD MEFA, PWD MEFA adjuvanted with dmLT, F18 FedF recombinant protein (as the positive control), or PBS as the control in adherence inhibition against F18 fimbrial bacteria 8516 to IPEC-J2 pig intestinal cells. (B) Serum samples from mice immunized with PWD MEFA, PWD MEFA adjuvanted with dmLT, K88 FaeG recombinant protein (as the positive control), or PBS as the control in adherence inhibition against K88+ ETEC strain 3030-2 to IPEC-J2 cells. The numbers of adherent bacteria (CFU) were converted to percentages, with CFU from cells treated with the control serum at 100%. (C) Mouse serum samples from two immunized groups (PWD MEFA and PWD MEFA with dmLT adjuvant) or the control in prevention of STa toxin from stimulation of cGMP in T-84 cells to show antibody neutralization activity against STa enterotoxicity. T-84 cells incubated with 2 ng STa toxin premixed with PBS, control mouse serum, or serum from each immunized group were measured for intracellular cGMP levels (pmol/ml) by using a cGMP EIA kit (Enzo Life). (D) Mouse serum samples from the two immunized groups (PWD MEFA and PWD MEFA with dmLT adjuvant) or the control in prevention of CT (LT homologue) from stimulation of cAMP in T-84 cells to show antibody neutralization activity against CT enterotoxicity. T-84 cells incubated with 10 ng CT toxin premixed with PBS, control mouse serum, or each immunized mouse serum were measured for intracellular cAMP levels (pmol/ml) by using a cAMP EIA kit (Enzo Life). ***, P < 0.001; **, P < 0.01.

Journal: Applied and Environmental Microbiology

Article Title: Application of a Novel Epitope- and Structure-Based Vaccinology-Assisted Fimbria-Toxin Multiepitope Fusion Antigen of Enterotoxigenic Escherichia coli for Development of Multivalent Vaccines against Porcine Postweaning Diarrhea

doi: 10.1128/AEM.00274-20

Figure Lengend Snippet: Mouse serum antibody in vitro neutralization activities against adherence of K88 fimbrial or F18 fimbrial bacteria and enterotoxicity of STa and CT toxins. (A) Serum samples from mice immunized with PWD MEFA, PWD MEFA adjuvanted with dmLT, F18 FedF recombinant protein (as the positive control), or PBS as the control in adherence inhibition against F18 fimbrial bacteria 8516 to IPEC-J2 pig intestinal cells. (B) Serum samples from mice immunized with PWD MEFA, PWD MEFA adjuvanted with dmLT, K88 FaeG recombinant protein (as the positive control), or PBS as the control in adherence inhibition against K88+ ETEC strain 3030-2 to IPEC-J2 cells. The numbers of adherent bacteria (CFU) were converted to percentages, with CFU from cells treated with the control serum at 100%. (C) Mouse serum samples from two immunized groups (PWD MEFA and PWD MEFA with dmLT adjuvant) or the control in prevention of STa toxin from stimulation of cGMP in T-84 cells to show antibody neutralization activity against STa enterotoxicity. T-84 cells incubated with 2 ng STa toxin premixed with PBS, control mouse serum, or serum from each immunized group were measured for intracellular cGMP levels (pmol/ml) by using a cGMP EIA kit (Enzo Life). (D) Mouse serum samples from the two immunized groups (PWD MEFA and PWD MEFA with dmLT adjuvant) or the control in prevention of CT (LT homologue) from stimulation of cAMP in T-84 cells to show antibody neutralization activity against CT enterotoxicity. T-84 cells incubated with 10 ng CT toxin premixed with PBS, control mouse serum, or each immunized mouse serum were measured for intracellular cAMP levels (pmol/ml) by using a cAMP EIA kit (Enzo Life). ***, P < 0.001; **, P < 0.01.

Article Snippet: Human colon carcinoma cell line T-84 (ATCC; CCL-248) and direct cAMP and cGMP EIA kits (Enzo Life Sciences, Inc., Farmingdale, NY) were used to measure mouse serum antibody neutralization activity against CT and STa enterotoxicity, as described previously ( 12 , 16 ).

Techniques: In Vitro, Neutralization, Recombinant, Positive Control, Inhibition, Activity Assay, Incubation

Supernatants of overnight grown culture (with an equal amount of cells) from each constructed strain were incubated with 1–2×10 5 T-84 cells. Panel A: Intracellular cGMP levels were measured using an EIA cGMP kit (Assay Designs), with 2 ng purified STa (from Robertson laboratory) was used as a positive control. Panel B: Intracellular cAMP levels were measured using an EIA cAMP kit with 10 ng CT as a positive control.

Journal: PLoS ONE

Article Title: Escherichia coli Expressing EAST1 Toxin Did Not Cause an Increase of cAMP or cGMP Levels in Cells, and No Diarrhea in 5-Day Old Gnotobiotic Pigs

doi: 10.1371/journal.pone.0043203

Figure Lengend Snippet: Supernatants of overnight grown culture (with an equal amount of cells) from each constructed strain were incubated with 1–2×10 5 T-84 cells. Panel A: Intracellular cGMP levels were measured using an EIA cGMP kit (Assay Designs), with 2 ng purified STa (from Robertson laboratory) was used as a positive control. Panel B: Intracellular cAMP levels were measured using an EIA cAMP kit with 10 ng CT as a positive control.

Article Snippet: To determine whether EAST1 possesses similar biological enterotoxicity, we examined field strain 17-2 (EAST1) and EAST1-positive recombinant strains for stimulation of cAMP or cGMP levels in human cell line T-84 (ATCC, CCL-248TM) and porcine epithelial cells line IPEC-J2 (a gift from Dr. Anthony Blikslager at North Carolina State University, Raleigh, NC) using an EIA cAMP ELISA kit and an EIA cGMP ELISA kit (Assay Designs, Ann Arbor, MI).

Techniques: Construct, Incubation, Purification, Positive Control

Intracellular cyclic AMP and GMP levels (pmole/ml) in T-84 cells incubated with culture supernatant of strains 8724 (EAST1/LT), 8607 (LT), 8725 (EAST1/STa), 8295 (STa), 17-2 (EAST1), or cell culture medium, measured using cAMP or cGMP EIA kits (Assay Design).

Journal: PLoS ONE

Article Title: Escherichia coli Expressing EAST1 Toxin Did Not Cause an Increase of cAMP or cGMP Levels in Cells, and No Diarrhea in 5-Day Old Gnotobiotic Pigs

doi: 10.1371/journal.pone.0043203

Figure Lengend Snippet: Intracellular cyclic AMP and GMP levels (pmole/ml) in T-84 cells incubated with culture supernatant of strains 8724 (EAST1/LT), 8607 (LT), 8725 (EAST1/STa), 8295 (STa), 17-2 (EAST1), or cell culture medium, measured using cAMP or cGMP EIA kits (Assay Design).

Article Snippet: To determine whether EAST1 possesses similar biological enterotoxicity, we examined field strain 17-2 (EAST1) and EAST1-positive recombinant strains for stimulation of cAMP or cGMP levels in human cell line T-84 (ATCC, CCL-248TM) and porcine epithelial cells line IPEC-J2 (a gift from Dr. Anthony Blikslager at North Carolina State University, Raleigh, NC) using an EIA cAMP ELISA kit and an EIA cGMP ELISA kit (Assay Designs, Ann Arbor, MI).

Techniques: Incubation, Cell Culture